TY  - JOUR
AB  - Integrins are a family of transmembrane proteins, involved in substrate recognition and cell adhesion in cross-talk with the extra cellular matrix. In this study, we investigated the influence of integrin α2β1 on tendons, another collagen type I-rich tissue of the musculoskeletal system. Morphological, as well as functional, parameters were analyzed in vivo and in vitro, comparing wild-type against integrin α2β1 deficiency. Tenocytes lacking integrin α2β1 produced more collagen in vitro, which is similar to the situation in osseous tissue. Fibril morphology and biomechanical strength proved to be altered, as integrin α2β1 deficiency led to significantly smaller fibrils as well as changes in dynamic E-modulus in vivo. This discrepancy can be explained by a higher collagen turnover: integrin α2β1-deficient cells produced more matrix, and tendons contained more residual C-terminal fragments of type I collagen, as well as an increased matrix metalloproteinase-2 activity. A greatly decreased percentage of non-collagenous proteins may be the cause of changes in fibril diameter regulation and increased the proteolytic degradation of collagen in the integrin-deficient tendons. The results reveal a significant impact of integrin α2β1 on collagen modifications in tendons. Its role in tendon pathologies, like chronic degradation, will be the subject of future investigations.
AU  - Kronenberg, Daniel
AU  - Michel, Philipp
AU  - Hochstrat, Eva
AU  - Wei, Ma
AU  - Brinckmann, Jürgen
AU  - Müller, Marcus
AU  - Frank, Andre
AU  - Hansen, Uwe
AU  - Eckes, Beate
AU  - Stange, Richard
DA  - 2020-04-18
DO  - 10.17879/44029555934
KW  - tendon biology
KW  - collagen
KW  - integrin α2β1
LA  - eng
N1  - International Journal of Molecular Sciences 21 (2020) 8, 2835, 1-15
N1  - Finanziert durch den Open-Access-Publikationsfonds der Westfälischen Wilhelms-Universität Münster (WWU Münster).
N1  - Förderer: Deutsche Forschungsgemeinschaft / Projektnummer: 194347757
N1  - Funding organisation: Deutsche Forschungsgemeinschaft / Project number: 194347757
PY  - 2020-04-18
TI  - Increased Collagen Turnover Impairs Tendon Microstructure and Stability in Integrin α2β1-Deficient Mice
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-44019471735
Y2  - 2024-11-22T06:25:03
ER  -