TY  - JOUR
AB  - Scanning electron microscopy (SEM) takes advantage of distinct detectors to visualise secondary and back-scattering electrons. Here, we report an integrated approach that relies on these two detection methods to simultaneously acquire correlated information on plasma membrane topography and curvature-sensitive cytosolic protein localization in intact cell samples. We further provide detailed preparation and staining protocols, as well as a thorough example-based discussion for imaging optimisation. Collectively, the presented method enables rapid and precise analysis of cytosolic proteins adjacent to cellular membranes with a resolution of ~100 nm, without time-consuming preparations or errors induced by sequential visualisation present in fluorescence-based correlative approaches.
AU  - Begemann, Isabell
AU  - Keller, Ulrike
AU  - Nüsse, Harald
AU  - Klingauf, Jürgen
AU  - Galic, Milos
DA  - 2020-05-26
DO  - 10.17879/14019536445
KW  - scanning electron microscopy
KW  - SE/BSE
KW  - CLEM
KW  - immunogold labelling
KW  - curvature-sensitive protein
KW  - BAR domain
LA  - eng
N1  - Cells 9 (2020) 6, 1329, 1-12
N1  - Finanziert durch den Open-Access-Publikationsfonds der Westfälischen Wilhelms-Universität Münster (WWU Münster).
PY  - 2020-05-26
TI  - Parallel Acquisition of Plasma Membrane Ultrastructure and Cytosolic Protein Localisation in Cultured Cells via Correlated Immunogold SEM
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-73099677857
Y2  - 2024-11-21T17:34:34
ER  -