TY  - JOUR
AB  - (1) Background: Gastric cancer (GC) is the fourth leading cause of cancer-related deaths worldwide. Helicobacter pylori infection is a major risk factor, but other microbial species may also be involved. In the context of an earlier proteomics study of serum and biopsies of patients with gastroduodenal diseases, we explored here a simplified microbiome in these biopsies (H. pylori, Acinetobacter baumannii, Escherichia coli, Fusobacterium nucleatum, Bacteroides fragilis) on the protein level. (2) Methods: A cohort of 75 patients was divided into groups with respect to the findings of the normal gastric mucosa (NGM) and gastroduodenal disorders such as gastritis, ulcer, and gastric cancer (GC). The H. pylori infection status was determined. The protein expression analysis of the biopsy samples was carried out using high-definition mass spectrometry of the tryptic digest (label-free data-independent quantification and statistical analysis). (3) Results: The total of 304 bacterial protein matches were detected based on two or more peptide hits. Significantly regulated microbial proteins like virulence factor type IV secretion system protein CagE from H. pylori were found with more abundance in gastritis than in GC or NGM. This finding could reflect the increased microbial involvement in mucosa inflammation in line with current hypotheses. Abundant proteins across species were heat shock proteins and elongation factors. (4) Conclusions: Next to the bulk of human proteins, a number of species-specific bacterial proteins were detected in stomach biopsies of patients with gastroduodenal diseases, some of which, like those expressed by the cag pathogenicity island, may provide gateways to disease prevention without antibacterial intervention in order to reduce antibiotic resistance.
AU  - Aziz, Shahid
AU  - Rasheed, Faisal
AU  - Akhter, Tayyab Saeed
AU  - Zahra, Rabaab
AU  - König, Simone
DA  - 2022
DO  - 10.17879/82079675786
KW  - gastric cancer
KW  - gastritis
KW  - ulcer
KW  - proteomics
KW  - Helicobacter pylori
LA  - eng
N1  - Molecules 27 (2022) 17, 5410, 1-13
N1  - Finanziert durch den Open-Access-Publikationsfonds der Westfälischen Wilhelms-Universität Münster (WWU Münster).
N1  - Förderer: Pakistan Science Foundation / Projektnummer: PSF/res/C‐PINSTECH/Med (497)
N1  - Funding organisation: Pakistan Science Foundation / Project number: PSF/res/C‐PINSTECH/Med (497)
PY  - 2022
TI  - Microbial Proteins in Stomach Biopsies Associated with Gastritis, Ulcer, and Gastric Cancer
UR  - https://nbn-resolving.org/urn:nbn:de:hbz:6-12079627437
Y2  - 2024-11-22T04:49:38
ER  -