<jats:title>ABSTRACT</jats:title>

<jats:p>The pSETT4 vector integrates into the <jats:italic>Actinoplanes</jats:italic> sp. SE50/110 chromosome via the bacteriophage φC31 integrase and allows cloning of a gene of interest by Golden Gate assembly (BsaI). T4 terminators surround the expression cassette to isolate the transcriptional unit and to prevent antisense transcription. The system can be used in other <jats:italic>Actinomycetales</jats:italic> by exchanging the promoter.</jats:p>