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Nippes, Alina; Ackermann, Doreen ; König, Simone: Analysis of CoFGE experiments with Delta2D. In: Mercator Journal of Biomolecular Analysis H. Mercator Journal of Biomolecular Analysis. Oktober 2018, S. 1-45
Inhalt
1. Generation of a Pool and a Project
2. Import Process und Image Activation
2.1 Importing Gel Images
2.2 Image Attributes
2.2.1 Sample
2.2.2 Gel
2.2.3 Channel
4. Creating a Fused Image
5. Warping of Sample Gels
5.1 Import and Image Attributes
5.2 Warping Strategy
5.3 Visualization of Warping Results
6. Export of Coordinates
6.1 Export of Raw Data Coordinates
6.2 Export of Coordinates of Warped Gels
7. Referencing in Small Projects
8. Semi-quantitative Analysis
As the amount of standard protein used for referencing is known, the amount present for proteins of interest can be estimated. The normalized volume (%V) as well as the integrated grey volume without background (V) can be added to the quantitation tab...