Physico-chemical factors that determine tracer substance flux from or into sealed vesicular structures are examined. Flux amplitudes are dependent on the average volume of a vesicle, while flux rates depend on the average number of transmembrane channels per vesicle. Gating processes leading to channel opening and/or closing affect both amplitudes and rates. Averaging over inhomogeneities in vesicle size and channel density leads to an explicit expression for time-dependent tracer content. Means for experimentally determining all variable factors in this expression are discussed.